qPCR is a technique that has become indispensable in modern laboratories. This quantitative variant of the PCR technique is widely used for virus detection, yeast and bacterial identification, gene expression analysis, and the detection of copy number variations.
It is often overlooked that this powerful technique also has its weaknesses. One of the most critical pitfalls lies in the quality of the PCR primers and the interpretation of the results. Proper primer design is therefore essential to ensure a high-quality analysis.
This workshop aims to introduce you to the basic principles of effective primer design. Through targeted computer exercises and the use of various bioinformatics tools, we will explain step by step what to look out for.
Format
This interactive workshop lasts one full day.
Target audience
Researchers who want to work with qPCR.
Maximum group size
To be determined in mutual agreement.